.. currentmodule:: fretbursts.burstlib The "Data()" class ================== The :class:`Data` class is the main container for smFRET measurements. It contains timestamps, detectors and all the results of data processing such as background estimation, burst data, fitted FRET and so on. The reference documentation of the class follows. .. contents:: "Data()" class: description and attributes ------------------------------------------ A description of the :class:`Data` class and its main attributes. .. module:: fretbursts.burstlib .. autoclass:: Data Summary information ------------------- List of :class:`Data` attributes and methods providing summary information on the measurement: .. class:: Data .. autoattribute:: time_max .. autoattribute:: time_min .. autoattribute:: ph_data_sizes .. autoattribute:: num_bursts .. automethod:: burst_sizes .. automethod:: burst_sizes_pax_ich .. automethod:: burst_sizes_ich .. autoattribute:: burst_widths .. automethod:: ph_in_bursts_ich .. automethod:: ph_in_bursts_mask_ich .. automethod:: status .. autoattribute:: name .. automethod:: Name Analysis methods ---------------- The following methods perform background estimation, burst search and burst-data calculations: - :meth:`Data.calc_bg` - :meth:`Data.burst_search` - :meth:`Data.calc_fret` - :meth:`Data.calc_ph_num` - :meth:`Data.fuse_bursts` - :meth:`Data.calc_sbr` - :meth:`Data.calc_max_rate` The methods documentation follows: .. class:: Data .. automethod:: calc_bg .. automethod:: burst_search .. automethod:: calc_fret .. automethod:: calc_ph_num .. automethod:: fuse_bursts .. automethod:: calc_sbr .. automethod:: calc_max_rate Burst corrections ----------------- Correction factors ^^^^^^^^^^^^^^^^^^ The following are the various burst correction factors. They are `Data` properties, so setting their value automatically updates all the burst quantities (including `E` and `S`). .. class:: Data .. autoattribute:: gamma .. autoattribute:: leakage .. autoattribute:: dir_ex .. autoattribute:: chi_ch Correction methods ^^^^^^^^^^^^^^^^^^ List of :class:`Data` methods used to apply burst corrections. .. class:: Data .. automethod:: background_correction .. automethod:: leakage_correction .. automethod:: dither Burst selection methods ----------------------- :class:`Data` methods that allow to filter bursts according to different rules. See also :ref:`burst_selection`. .. class:: Data .. automethod:: select_bursts .. automethod:: select_bursts_mask .. automethod:: select_bursts_mask_apply Fitting methods ------------------- Some fitting methods for burst data. Note that E and S histogram fitting with generic models is now handled with the new :ref:`fitting framework `. .. class:: Data .. automethod:: fit_E_generic .. automethod:: fit_E_m .. automethod:: fit_E_ML_poiss .. automethod:: fit_E_minimize .. automethod:: fit_E_two_gauss_EM Timestamp access methods ------------------------ The following methods are used to access (or iterate over) the arrays of timestamps (for different photon streams), timestamps masks and burst data. - :meth:`Data.get_ph_times` - :meth:`Data.ph_in_bursts_ich` - :meth:`Data.ph_in_bursts_mask_ich` - :meth:`Data.iter_ph_times` - :meth:`Data.get_ph_mask` - :meth:`Data.iter_ph_masks` - :meth:`Data.iter_bursts_ph` - :meth:`Data.expand` - :meth:`Data.copy` - :meth:`Data.slice_ph` The methods documentation follows: .. class:: Data .. automethod:: get_ph_times .. automethod:: iter_ph_times .. automethod:: get_ph_mask .. automethod:: iter_ph_masks .. automethod:: iter_bursts_ph .. automethod:: ph_in_bursts_ich .. automethod:: ph_in_bursts_mask_ich .. automethod:: expand .. automethod:: copy .. automethod:: slice_ph