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Single-molecule FRET burst analysis
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**FRETBursts** is an open-source
python package for burst analysis of freely-diffusing
`single-molecule FRET <https://en.wikipedia.org/wiki/Single-molecule_FRET>`__
data for single and multi-spot experiments. FRETBursts supports both
single-laser and dual-laser alternated excitation
(`ALEX <https://doi.org/10.1073/pnas.0401690101>`__ and
`PAX <https://doi.org/10.1007/s00249-007-0133-7>`__)
as well as
`ns-ALEX <https://doi.org/10.1073/pnas.0508584102>`__ (or
`PIE <https://doi.org/10.1529/biophysj.105.064766>`__).

We provide well-tested implementations of state-of-the-art
algorithms for confocal smFRET analysis.
We focus on computational reproducibility,
by using `Jupyter notebook <http://jupyter.org/>`__ based interfaces.

Please send questions or report issue on `GitHub <https://github.com/OpenSMFS/FRETBursts/issues>`__.

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   <h2>Documentation</h2>

* `Introducing FRETBursts <http://tritemio.github.io/smbits/2016/02/19/fretbursts>`__
* :doc:`Installation <getting_started>`
* :doc:`What's new? <releasenotes>`
* `μs-ALEX Tutorial <http://nbviewer.jupyter.org/github/OpenSMFS/FRETBursts_notebooks/blob/master/notebooks/FRETBursts%20-%20us-ALEX%20smFRET%20burst%20analysis.ipynb>`__
* `List of Jupyter Notebooks <https://github.com/OpenSMFS/FRETBursts_notebooks#fretbursts-notebooks>`__
* :doc:`Reference manual <reference_manual>`

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   <h2>Features</h2>

* `FRETBursts Paper <http://dx.doi.org/10.1101/039198>`__
* :doc:`Burst Search Algorithm <burstsearch>`
* `BVA <http://nbviewer.jupyter.org/github/OpenSMFS/FRETBursts_notebooks/blob/master/notebooks/Example%20-%20Burst%20Variance%20Analysis.ipynb>`__
* `2CDE <http://nbviewer.jupyter.org/github/OpenSMFS/FRETBursts_notebooks/blob/master/notebooks/Example%20-%202CDE%20Method.ipynb>`__
* `Exporting burst data <http://nbviewer.jupyter.org/github/OpenSMFS/FRETBursts_notebooks/blob/master/notebooks/Example%20-%20Exporting%20Burst%20Data%20Including%20Timestamps.ipynb>`__
* `Report an issue <https://github.com/opensmfs/FRETBursts/issues>`__